Molecular Epidemiology and Mechanism of Producing ?-Lactamases in Multidrug-resistant Acinetobacter
- VernacularTitle:多药耐药不动杆菌属流行病学调查及产酶机制研究
- Author:
Xiaoman AI
;
Xiuzhen ZHANG
;
Yunjian HU
- Publication Type:Journal Article
- Keywords:
Acinetobacter;
Multidrug resistance;
Carbapenemase
- From:
Chinese Journal of Nosocomiology
2009;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the variation of in vitro activity,the ?-lactamases,type diversity and the homology of multiple resistances in Acinetobacter isolated.METHODS The multiple resistant Acinetobacter were selected to detect susceptibility test by K-B antimicrobial agents.The resistant rates were analyzed by WHONET 5.4,the isolates ?-lactamases phenotype was detected by three-dimensional test,genomic types were measured by PFGE.The ?-lactamases genotype was determined by PCR assay with specific-primer,and DNA sequencing was also used to analyze resistance-related gene.RESULTS Twenty-eight of 45 strains were OXA producing strains(68.3%),10 strains were IMP producing strains(24.4%),13 strains were TEM producing strains(31.7%),18 strains were CTX-M producing strains(43.9%),6 strains were PER producing strains(14.6%),and 7 strains were AmpC producing strains(17.1%).None produced SHV ?-lactamases.Twenty-four strains were produced 2 or more than 2 kinds of ?-lactamases.CONCLUSIONS The multiple resistance of Acinetobacter can produce kinds of ?-lactamases,but producing ?-lactamases are not the only one mechanism.
