Aminoglycoside-modifying Enzyme and 16S rRNA Methylase Gene Expressions in Acinetobacter baumannii
- VernacularTitle:新疆地区鲍氏不动杆菌氨基糖苷类修饰酶与16S rRNA甲基化酶基因研究
- Author:
Ping JI
;
Yumei LIU
;
Yan JIANG
;
Zhaoxia ZHANG
- Publication Type:Journal Article
- Keywords:
Aminoglycoside-modifying enzyme gene;
16S rRNA methylase gene;
Acinetobacter baumannii
- From:
Chinese Journal of Nosocomiology
2009;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To understand aminoglycoside-modifying enzyme and 16S rRNA methylase gene expressions in Acinetobacter baumannii in Xinjiang region.METHODS 20 A.baumannii strains were isolated and test the anti-bacterial drug sensitivity.PCR methods were used to test aminoglycoside-modifying enzyme and 16S rRNA methylase gene.RESULTS From thirteen A.baumannii strains detected the aminoglycoside-modifying enzyme genes,the detection rate was 65%;in which aac(3)-Ⅰ gene was positive in 4 strains(20%),aac(3)-Ⅱ gene in 8 strains(40%),aac(6′)-Ⅰad gene in 4 strain(20%),ant(3″)-Ⅰ gene in 4 strain(20%),and ant(2″)-Ⅰ gene was positive in 1 strain(5%).The aac(6′)-Ⅰ b and aac(6′)-Ⅱ gens and were not detected;the 16S rRNA gene methylation was negative.CONCLUSIONS There are aminoglycoside-modifying enzyme genes existing in A.baumannii,no 16S rRNA methylase gene was detected in Xinjiang region.
