Expressions of myocardial ?-MHC and ?-MHC mRNA in rats with pressure overload
- VernacularTitle:压力负荷增加大鼠模型心肌?-MHC和?-MHC mRNA的表达
- Author:
Hui CAI
;
Junhao GUO
;
Wei SHANG
;
Qunyan ZHANG
- Publication Type:Journal Article
- Keywords:
Myocardial hypertrophy;
?-MHC;
?-MHC;
Gene expression;
Pressure overload
- From:
Journal of Medical Postgraduates
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Recent years has witnessed new progress in the molecular genetic study of the relationship between the myosin heavy chain and myocardial hypertrophy.This study aimed to observe the expressions of myocardial ?-myosin heavy chain(?-MHC) and ?-myosin heavy chain(?-MHC) mRNA in rats with pressure overload,and to investigate the mechanism by which abdominal aorta constriction induces left ventricular hypertrophy.Methods: Male SD rats were randomly divided into a sham operation and model group.The models were made by clamping the suprarenal abdominal aorta with a silver chip.Eight weeks later,changes in the left ventricular mass index(LVMI) and the ultra microstructure of the left ventricular myocyte were observed,and the expressions of ?-MHC and ?-MHC in the myocardial tissue were detected by RT-PCR.Results: Compared with the sham operation group,the model rats showed markedly increased LVMI(P
