Analysis of Cellulase Regulator ACEII Interaction with cbh1 Promoter Fragment in Trichoderma koningii
- VernacularTitle:康氏木霉纤维素酶转录因子ACEII与外切纤维二糖水解酶基因I(cbh1)启动子的结合分析
- Author:
Min LING
;
Gang LIANG
;
Yongling QIN
;
Nan LI
;
Zhiqun LIANG
- Publication Type:Journal Article
- Keywords:
ACEII Cellulase cbh1 Electrophoresis mobility shift assay Trichoderma koningii
- From:
China Biotechnology
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
ACEI,ACEII and Xyr1 are transcriptional factors that regulate cellulase gene expression in Trichoderma koningii.In vitro experiments have shown that ACEI and Xyr1 bind to the cbh1 promoter fragment(-304 to-18) and regulate the gene transcription.However,whether ACEII binds to this 287bp fragment is still unclear.To further elucidate the regulatory mechanism of ACEII for cellulase,DNA-binding domains of ACEII from T.Koningii were expressed in E.coli.It could not show binding to the cbh1 promoter fragment(-304 to-18) by electrophoresis mobility shift assays,suggesting that it is Xyr1 but not ACEII binds playing an essential role during induction of cbh1 gene transcription.
