Overexpression and Effect on Apoptosis of the 150-ku Oxygen-regulated Protein(ORP150) in Human Hepatocellular Carcinoma
- VernacularTitle:氧调节蛋白150在人肝细胞癌中的过度表达及其对凋亡的作用(英文)
- Author:
Haijun ZHOU
;
Zhenyu HEI
;
Jiong SHI
;
Kun GUO
;
Bingsheng SUN
;
Jincai WU
;
Yue ZHAO
;
Liyun FU
;
Chun DAI
;
Dongmei GAO
;
Ruixia SUN
;
Yan ZHAO
;
Jie CHEN
;
Lu WANG
;
Lunxiu QIN
;
Yinkun LIU
- Publication Type:Journal Article
- Keywords:
150-ku oxygen-regulated protein(ORP150), human hepatocellular carcinoma, apoptosis
- From:
Progress in Biochemistry and Biophysics
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
In previous study, the 150-ku oxygen-regulated protein(ORP150) was identified as a candidate glycoprotein related to hepatocellular carcinoma.In order to further validate the expression level of ORP150 in hepatocellular carcinoma, protein expression was determined by Western blot and cell immunochemistry, and messenger RNA(mRNA) expression was detected by quantitative real-time polymerase chain reaction.The effect of ORP150 on apoptosis and invasive potential of hepatocellular carcinoma cells was evaluated using the small interference RNA(siRNA) technique.Both the protein and mRNA expression levels of ORP150 were significantly upregulated in hepatocellular carcinoma cell lines compared with a non-tumor human liver cell line.After transfection with the specific siRNA of ORP150, significantly greater apoptosis of hepatocellular carcinoma cells was induced compared with untransfected cells.However, no significant effect on invasive potential was found.Overexpression of ORP150 was associated with hepatocellular carcinoma, and ORP150 might promote the proliferation of hepatocellular carcinoma cells by inhibiting apoptosis.ORP150 could be a potential therapeutic target for hepatocellular carcinoma.
