Drug-resistant Genes aadA4/5 and aph(3′)-Ⅰ Associated with Aminoglycosides Be Found Existing in Escherichia coli Isolated from ICU
- VernacularTitle:大肠埃希菌ICU分离株发现aadA4/5、aph(3′)-Ⅰ型氨基糖苷类修饰酶基因
- Author:
Guozhong CHEN
;
Yiping WANG
;
Jianfei YING
;
Yanhong YU
;
Mingyang HE
;
Minfei AN
;
Chengjie ZHOU
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Aminoglycosides;
Drug-resistant genes;
Aminoglycoside-modifying;
enzymes 16S RNA methylase;
aadA4/5 aph(3′)-Ⅰ
- From:
Chinese Journal of Nosocomiology
2009;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the antibiotics resistance of Escherichia coli(ECO) and distrubtion of aminoglycoside-modifying enzymes and 16S RNA methylase genes in ICU.METHODS The samples of 20 ECO isolates were collected from Dec 2007 to Jun 2008 of patients in ICU.To determine the sensitivity to the 30 antibacterials K-B method was used and aminoglycoside-modifying enzymes and 16S RNA methylase genes were analyzed by polymerase chain reaction(PCR).RESULTS In among the 20 ECO isolates,8 strains carried aac(3)-Ⅱ(40%),3 carried aac(6′)-Ⅰb(15%) and ant(3″)-Ⅰ(15%),1 be found aph(3′)-Ⅰ(10%)and 13 be found aadA4/5 aminoglycoside-modifying enzymes genes,no strain carried 16S RNA methylase genes.CONCLUSIONS aac(3)-Ⅱ、aac(6′)-Ⅰb,ant(3″)-Ⅰ,aph(3′)-Ⅰ and aadA4/5 aminoglycoside-modifying enzymes genes exist in ECO widely,they should be the main cause inducing the high rate of drug-resistance to aminoglycosides.
