Diagnostic Value of Detection of 16S rRNA Gene of Pathogens from Blood by PCR
- VernacularTitle:PCR在血液病原菌16S rRNA基因检测的诊断价值
- Author:
Changfa YU
;
Lijun YE
;
Yingpeng REN
;
Darong DUAN
;
Ronghua RUAN
;
Xiansen ZHANG
- Publication Type:Journal Article
- Keywords:
16S rRNA;
Polymerase chain reaction;
Pathogen
- From:
Chinese Journal of Nosocomiology
2009;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore study a method for rapid detection of bacterial infection in clinic to diagnose septicemia early.METHODS 16S rRNA gene of ten bacterial species was amplified with PCR,by using human genome DNA,HBV-DNA and Candida albicans as comparison.The sensitivity test was done by the method of gradual dilution of Escherichia coli.RESULTS The bacterial species were amplified and the products were 371 bp,but human genome DNA,HBV-DNA and C.albicans showed no amplification products.Sensitivity test showed that it could detect as low as 1.5?104/L of E.coli.CONCLUSIONS The method is rapid and highly specific and sensitive in detecting the existence of bacterial 16S rRNA gene.
