Drug-resistant Genes Associated with Beta-lactamases in Acinetobactor baumannii Isolated from ICU
- VernacularTitle:ICU鲍氏不动杆菌分离株?-内酰胺酶基因研究
- Author:
Yiping WANG
;
Guozhong CHEN
;
Jianfei YING
;
Yanhong YU
;
Mingyang HE
;
Minfei AN
;
Chengjie ZHOU
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Beta-lactamases;
ESBLs;
Metallo-?-lactamases;
AmpC-?-lactamases;
TEM-116 Type gene;
OXA-73 Carbapenemase
- From:
Chinese Journal of Nosocomiology
2009;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the antibiotics resistance of multi-resistant Acinetobactor baumannii(ABA) and genotypes of beta-lactamases in ICU.METHODS The samples of 20 A.baumannii isolates were collected from Oct 2007 to Jul 2008 from patients in ICU.To determine the sensitivity to the 32 kinds of antibacterials,K-B method was used and the detection of ESBLs and AmpC beta-lactamases was performed by three dimensional test and 21 types of beta-lactamases genes were analyzed by polymerase chain reaction(PCR).RESULTS Among the 20 ABA isolates,all carried TEM beta-lactamases gens(100%),10 carried OXA-23 beta-lactamases gens(50%) and 15 strains carried ADC beta-lactamases gene(75%),50% strains produced TEM,OXA-23 and ADC beta-lactamases simultaneously.Through determining sequence of one PCR product from TEM,OX23 and ADC respectively,we found TEM-116,OXA-73 and ADC-25 type beta-lactamases genes.CONCLUSIONS The antibiotics resistance of ABA is very serious.TEM,OXA-23 and ADC exist in multi-resistant A.baumannii widely.It should be the main causes as high rate of drug-resistantce to beta-lactamantibiotics.
