Construction of eukaryotic expression recombinant plasmid of human fibrinolysin
- VernacularTitle:人纤溶酶真核表达重组质粒的构建
- Author:
Yang WU
;
Yuqin LI
;
Zhihao WANG
;
Xiuxian ZANG
- Publication Type:Journal Article
- Keywords:
human fibrinolysin;
anti-thrombus;
eukaryotic expression vector;
recombinant plasmid
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the eukaryotic expression vector of human fibrinolysin for further study on anti-thrombus and thrombolysis.Methods Human fibrinolysin gene was amplified from human fetal liver tissue with PCR,and was cloned into eukaryotic expression plasmid pCI-neo and then sequenced.After the correct identification by sequencing,the eukaryotic expression recombinant plasmid of human fibrinolysin was constructed and identified with PCR and enzyme digestion.Results The PCR amplification product of fibrinolysin gene was 1 740 bp.The result of sequencing was the same as that registered in GenBank.The results of PCR and enzyme digestion showed that the recombinant eukaryotic expression plasmid had correct codogenic gene fragment.Conclusion The eukaryotic expression recombinant plasmid of human fibrinolysin gene is successfully constructed and identified.
