Adipose Gene Expression Profiles Related to Metabolic Syndrome Using Microarray Analyses in Two Different Models.
10.4093/dmj.2014.38.5.356
- Author:
Hye Jin YOO
1
;
Hwan Jin HWANG
;
Tae Woo JUNG
;
Ja Young RYU
;
Ho Cheol HONG
;
Hae Yoon CHOI
;
Sei Hyun BAIK
;
Kyung Mook CHOI
Author Information
1. Division of Endocrinology and Metabolism, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea. medica7@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Lipocalin-2;
Microarray;
PPAR gamma
- MeSH:
Adipocytes;
Adipokines;
Animals;
Body Mass Index;
Carrier Proteins;
Genes, Overlapping;
Humans;
Interleukin-6;
Intra-Abdominal Fat;
Leukocyte Count;
Mice;
Microarray Analysis;
Models, Animal;
Obesity;
Peroxisomes;
Polymerase Chain Reaction;
PPAR gamma;
Rats;
Rats, Inbred OLETF;
Reverse Transcription;
Transcriptome*
- From:Diabetes & Metabolism Journal
2014;38(5):356-365
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist has a wide-ranging influence on multiple components of metabolic syndrome. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is a useful animal model of metabolic syndrome. To determine genes related to metabolic syndrome, we examined overlapping genes that are simultaneously decreased by PPAR-gamma agonists and increased in OLETF rats using microarrays in two different models. METHODS: In the first microarray analysis, PPAR-gamma agonist-treated db/db mice were compared to standard diet-fed db/db mice. In the second microarray analysis, OLETF rats were compared to Long-Evans Tokushima Otsuka (LETO) rats (control of OLETF rats). RESULTS: Among the overlapping genes, in the present study, we validated that lipocalin-2 expression was significantly decreased in the visceral adipose tissue of PPAR-gamma agonist-treated db/db mice compared to standard diet-fed db/db mice and increased in OLETF rats compared to LETO rats using real time reverse transcription polymerase chain reaction. Furthermore, we showed for the first time that lipocalin-2 expression was significantly increased in the visceral adipose tissues of obese humans compared with nonobese humans. In addition, the expression level of lipocalin-2 in human visceral adipose tissue had a significant positive correlation with body mass index, serum interleukin-6, adipocyte fatty acid binding protein levels, and white blood cell count. CONCLUSION: Lipocalin-2 was confirmed to be a significant adipokine affected by PPAR-gamma agonist and obesity in the present study. Also, for the first time in human visceral adipose tissue, it was determined that the expression of lipocalin-2 from obese humans was significantly increased and correlated with circulating inflammatory markers.
