16S rRNA methylase genes in ESBLs-producing Enterobacteriaceae
10.3760/cma.j.issn.1674-2397.2010.03.004
- VernacularTitle:产ESBLs肠杆菌科细菌中16S rRNA甲基化酶基因的研究
- Author:
Xiaoqiang SHEN
;
Hua ZHOU
;
Shujuan JI
;
Zeqing WEI
;
Jianpu XU
;
Yunsong YU
;
Lanjuan LI
- Publication Type:Journal Article
- Keywords:
Beta-lactamases;
Gene;
16S rRNA methylase;
Drug resistance;
Plasmid
- From:
Chinese Journal of Clinical Infectious Diseases
2010;3(3):142-144,165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prevalence and dissemination mechanism of 16S rRNA methylase genes in extended-spectrum beta-lactamases(ESBLs)-producing Enterobacteriaceae in China.Methods PCR amplification and DNA sequencing were used for screening and identifing 16S rRNA methylase genes and ESBLs genes.Minimal inhibitory concentrations(MICs)of the antimicrobial agents were detected by Etest.Conjugation and plasmid extract were performed to study dissemination mechanism of 16S rRNA methylase genes and ESBLs genes.Results Only one strain.Klebsiella oxytoca strain ZJ157 was screened as positive for armA gene from 447 ESBLs-producing isolates,which also contained CTX-M-15 and TEM-1 genes.It was resistant to aminoglycesides,ciprofloxacin,and most β-lactams,except carbapenems,polymyxin E and tigecyeline.Resistance to amikacin and β-lactams was transferred to a recipient Escherichia coli 600 by conjugation experiment.arntA.CTX-M-15 and TEM-1 genes were detected in the transconjugant.A plasmid about 55 kb was extracted from Klebsiella oxytoca ZJl57 and the transconjugant.Conclusions A 16S rRNA methylase gene armA was detected in an isolate of Klebsiella oxytoca.armA,CTX-M-15 and TEM-1 genes can be co-transferred in the same plasmid leading to multi-drug resistance.
