Effect of extrinsic vascular endothelial growth factor on viability of skin flap treated with radiotherapy in rat at different time after operation
- VernacularTitle:术后不同时间给予外源性VEGF对大鼠放疗皮瓣成活的影响
- Author:
Chunli LIU
;
Yue WANG
;
Yanhai CHE
- Publication Type:Journal Article
- Keywords:
vascular endothelial growth factor;
radiotherapy;
sink flap;
rats,wistar
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of vascular endothelial growth factor(VEGF) on the viability of skin flap treated with radiotherapy at different time after operation and clarify its enhancing effect on angiogenesis.Methods Fifty Wistar rat were randomly divided into 3,5,7,9 d groups and control group.A 3 cm?4 cm full thickness dorsal flap with the pedicle remaining attached at the posterior end in rats and were treated with 60Co radiotherapy at the 2nd,4th,and 6th day postoperatively.The total quantity was 12 Gy.In the experiment groups every rat was given VEGF 120 ng on the 3rd,5th,7th and 9th day.The rats in control group were given the same quality normal saline and radiotherapy.Pathological observation,fluorescence staining and sesuccinic dehyrogenase(SDH) assay were used to measure the blood vessel density,blood vessel diameter,microcirculation and cell vitality of skin flap.Results In 5 d and 7 d VEGF group,the number of average blood vessels and cells of the skin falp was increased compared with control group(P
