Effect of interleukin-15 in pathogenesis of mouse bronchial asthma
- VernacularTitle:白细胞介素15在小鼠支气管哮喘发病中的作用
- Author:
Kaishu ZHAO
;
Yunfeng ZHANG
;
Shijie GUO
;
Yanzhi HUANG
;
Hang GAO
;
Jirong LU
- Publication Type:Journal Article
- Keywords:
asthma;
disease models,animal;
interleukin-15;
Th1/Th2;
eosinophil;
glucocorticoid
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the level of interleukin-15(IL-15) in serum and its expression in lung tissues,and analyze the correlations between IL-15 and IL-4,IFN-?,eosinophil(Eos),and explore the effect of IL-15 on bronchial asthma.Methods Thirty femal BALB/C mice were randomly divided into three group(n=10),group A(asthma model),group B(corticosteroid treatment) and group C(normal control).All mice were killed 24 h after final OVA challenge.Blood were obtained for measurement of serum IgE,IL-4,IFN-? and IL-15 levels by enzyme-linked immunoabsorbent assay(ELISA).Bronchoalveolar lavage fluid(BLAF) was collected for Eos count.The left lungs were isolated for pathological examination.The lung sections were stained with hematoxylin and eosin(HE).The expressions of IL-15 in lung tissues were measured by immunohistochemical SP method.Results ①The mouse asthma model appeared ethological changes specific to asthma,the Eos count in BALF was increased,and IgE and IL-4 levels in serum were also increased compared with control group(P
