RNAi inhibits Survivin expression and increases cell apoptosis
- VernacularTitle:RNAi抑制Survivin基因表达增加细胞凋亡
- Author:
Fang XUE
;
Mingying DUAN
;
Yan CHEN
- Publication Type:Journal Article
- Keywords:
RNA interference;
Survivin;
caspase 3/7
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of down regulation of SURVIVIN on cell and subsequent apoptosis in cervical cancer cells HeLa.Methods(1)The U6 promoter was obtained by PCR from liver of mouse and ligated into TA vector.RNAi vector(psSURVIVIN) and psN(control vector) containing of the U6 promoter was established.(2)Using HeLa cells as a model system,two groups were set stably up transfected with RNAi control plamid and psSURVIVIN(SURVIVIN RNAi plasmid),respectively.The expression of SURVIVIN in HeLa cells was measured by Western blot and immunofluorescence methods.(3)The activity of caspase 3/7 was detected using caspase-Glo(tm) 3/7 assay reagent.Results(1)Cell apoptotic rate was significantly increased by transfection with RNAi targeting plasmid,and cell was arrested at G0/G1(P
