Dimethyl sulfoxide induces bone marrow mesenchymal stem cells differentiation into cardiomyocyte-like cells in vitro
- VernacularTitle:二甲基亚砜体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞
- Author:
Haiping WANG
;
Lei ZHANG
;
Suxia SHAO
;
Jing ZHAO
- Publication Type:Journal Article
- Keywords:
marrow mesenchymal stem cells;
dimethyl sulfoxide;
cardiomyocytes;
rat
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the optimal condition for the differentiation of rat bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte-like cells in vitro by using DMSO.Methods SD rat MSCs were isolated from rat bone marrow and cultured,then induced by 1.0% DMSO for 24 h.The cultured cells were observed through phase-contrast microscopy.Immunohistochemical technique and laser scanning confocal microscopy(LSCM) were used for detecting the expression of desmin,?-sarcomeric actin and C-TnT.The ultrastructures were examined with transmission electron microscopy.Results MSCs induced by DMSO were identified by the positive staining for desmin,?-sarcomeric actin and C-TnT.Transmission electron microscopy showed that paralleled myofilaments and a lot of rough endoplasmic reticulum were formed.Conclusion MSCs can be induced to differentiate into cardiomyocyte-like cells by DMSO.
