Effect of 8-nitrochyrsin on the proliferation and apoptosis of HL-60 cells
- VernacularTitle:8-硝基白杨素抑制HL-60细胞系增殖及促凋亡
- Author:
Jun YUE
;
Shihua WU
- Publication Type:Journal Article
- Keywords:
human acute myeloid leukaemia;
chrysin;
8-nitrochyrsin;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 8-Nitrochyrsin on cell proliferation and cell apoptosis of HL-60 cells in vitro.Methods 8-Nitrochyrsin on proliferation of HL-60 cells was detected by MTT assay;The trypan blue exclusion method was used to count the number of viable and dead cells,then the survival rate and growth curve were achieved;DNA ladder bands were observed by DNA agarose gel electrophoresis;HL-60 cell apoptotic percentage was detected by flow cytometry with PI staining.Results 8-Nitrochrysin inhibited proliferation of HL-60 cells in a concentration-dependent manner;8-Nitrochyrsin possess the inhibitory effect on the proliferation and growth of HL-60 cells;8-Nitrochrysin possess the significantly effect of inducing apoptosis of HL-60 cells.Conclusion 8-Nitrochyrsin possess the inhibitory effect of the proliferation and growth of HL-60 cells and significantly induces apoptosis of HL-60 cells.
