Detection of Two Types of Aminoglycoside-modifying Enzymes Genes in Chryseobacterium spp
- VernacularTitle:金黄杆菌属检出aac(6′)-Ⅱ和ant(2″)-Ⅰ耐药基因研究
- Author:
Desong MING
;
Zhishan ZHANG
;
Jianliang ZHUANG
;
Zhijun SU
;
Zunjin XIE
- Publication Type:Journal Article
- Keywords:
Chryseobacterium spp;
Aminoglycoside-modifying enzymes;
aac(6′)-Ⅱ;
ant(2″)-Ⅰ
- From:
Chinese Journal of Nosocomiology
2009;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study 6 kinds of aminoglycoside-modifying enzymes (AMEs) genes in Chryseobacterium spp isolates. METHODS The isolates were identified by API20NE Gram-negative identification cards,and the susceptibility of antimicrobial agents was detected by MIC kits ( bioM?rieux ) 6 AMEs genes of 2 strains of Chryseobacterium spp were measured by PCR,and verified by DNA sequencing and sequence analysis. RESULTS In the 2 strains,2 kinds of resistant genes [aac(6′)-Ⅱ and ant(2″)-Ⅰ] were positive,and 4 kinds genes of AMEs [aac (6′)-Ⅰb,aac(3)-Ⅱ,ant(3″)-Ⅰ and aac(3)-Ⅰ] were negative.The amplicons were purified,sequenced and analyzed with BLAST 2.0 and found to be identical to aac(6′)-Ⅱ and ant(2″)-Ⅰ. CONCLUSIONS There are AMEs in Chryseobacterium spp isolates. This is the first report on AMEs genes [coexistance of aac(6′)-Ⅱ and ant(2″)-Ⅰ] in Chryseobacterium spp.
