Transinfection of rabbit chondrocytes via chitosan microsphere as gene carriers with recombined human IL-1Ra gene and TGF-β1 gene
10.3760/cma.j.issn.1001-2036.2009.06.014
- VernacularTitle:壳聚糖微球介导人IL-1Ra与TGF-β1基因转染兔软骨细胞的研究
- Author:
Xiaoyue WEN
;
Daozhang CAI
;
Huading LU
;
Chun ZENG
;
Bin LIU
- Publication Type:Journal Article
- Keywords:
Chitosan;
Microsphere;
Interleukin-1 receptor antagonist;
Transforming growth factor β1;
Gene transinfection;
Cartilage
- From:
Chinese Journal of Microsurgery
2009;32(6):471-473,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore transinfection of rabbit chondrocytes via chitosan microsphere with human IL-1Ra and TGF-β1 gene. Methods Chitosan-DNA microspheres carrying plasmids with IL-1 Ra and TGF-β1 genes were prepared.The encapsulation efficiency,DNA-released kinetics and lysozyme degradation in vitro were performed.Articular rabbit chondrocytes were co-transinfected with the plasmids with IL-1Ra and TGF-β1 genes via chitosan-DNA mierosphere,evaluated by fluorescence microscope,TaqMan real-time PCR assay and MTF test. Results The chitosan microspheres with IL-1Ra and TGF-β1 genes were(2.8±0.2)μm and(2.6±0.1)μm in diameters respectively.The encapsulation efficiency were(88.3±4.1)%and(87.2±2.6)%.During the degradation,significant morphological changes were noticed.The plasmids could be released in a multiphasic fashion.Enhanced green fluorescent protein and Real-Time PCR analysis showed that genes were expressed in chondrocytes.lasting near 30 days.MTT indicated that the cotransinfection promoted the chondrocytes'proliferation. Conclusion IL-1Ra and TGF-β1 genes cotransfected into chondrocytes via chitosan-DNA microsphere could be expressed in a long term and cotransinfection promoted the chondroeytes'proliferation,which is the base of inhibiting the degeneration of cartilage and promote cartilage repair.
