Detection of 16S rRNA methylase genes in Klebsiella pneumoniae
- VernacularTitle:肺炎克雷伯菌中16S rRNA甲基化酶基因的研究
- Author:
Yinmei YANG
;
Canhua CHEN
;
Wei WANG
;
Xiaomian ZHOU
;
Huifen YE
;
Huiling CHEN
- Publication Type:Journal Article
- Keywords:
aminoglycoside;
16S rRNA methylase gene;
Klebsiella pneumoniae
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prevalence of 16S rRNA methylase genes in Klebsiella pneumoniae isolates from Guangzhou.Methods K-B test was used to determine the resistant rates of these stains.Five 16S rRNA methylase genes,armA,rmtA,rmtB,rmtC,and rmtD,were detected by PCR.Results All 55 K.pneumoniae isolates showed resistant to arbekacin,gentamicin,tobramycin,and neomycin.Susceptibility rates were 5.5%,20.0%,72.7%,and 100% to ceftazidime,ciprofloxacin,piperacillin/tazobactam,and imipenem respectively.ESBLs were positive in 52 of 55 (94.5%) isolates.Among 55 K.pneumoniae isolates,34 were positive for armA and 1 for rmtB.Conclusions In K.pneumoniae resistant to arbekacin,the positive rate of 16S rRNA methylase genes was high,predominantly with armA positive.These strains were highly resistant to some antibiotics.
