Expression and clinical significance of the genes associated with TGF-?/Smad signaling pathways in peripheral blood mononuclear cells of rheumatoid arthritis patients
- VernacularTitle:TGF-?/Smad信号转导通路相关基因在RA患者PBMC中的表达及其临床意义
- Author:
Liwen WANG
;
Chunyan LIU
;
Yun WANG
- Publication Type:Journal Article
- Keywords:
RA;
TGF-?/Smad signaling pathways;
TGF-? RII;
Smad4;
Smad7;
Smad4/Smad7
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of genes associated with TGF-?/Smad signaling pathways in peripheral blood mononuclear cells(PBMC)of rheumatoid arthritis(RA)patients and to evaluate the relevant clinical significance.Methods Conventional and nested SYBR green I fluorescent dye real-time PCR was used to detect the expression of TGF-? RII,Smad4,and Smad7 mRNA.Results Compared with those of 29 healthy persons,the expressions of TGF-? RII and Smad4 mRNA,and the ratio of Smad4/Smad7 in PBMC of 58 RA patients were significantly increased (P
