Glutaral Solution Conternts Determined by Enzyme-labeled Device
- VernacularTitle:酶标仪测定戊二醛溶液含量
- Author:
Yuqing XIAO
;
Xiaobo XIAO
;
Haixia HU
;
Mei CAO
;
Shigang YOU
;
Ping XIAO
- Publication Type:Journal Article
- Keywords:
Enzyme-labeled device;
Fehling reagent;
Glutaral;
Assaying
- From:
Chinese Journal of Nosocomiology
2009;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the analytic method for assaying of glutaral concentration by enzyme-labeled device at one time.METHODS Controlled solution of glutaral and sample solution were taken out and kept until the room temperature.Optical density value was detected at 450 nm.Content of sample was calculated by comparison with the known concentration of glutaral.RESULTS In the range of 0.75-2.50%,concentration of glutaral had linear relationship with the optical density value.Reproductive test:relative average error was within 2%,RSD≤2.51%;interference test:oxidation-resistant-sodium nitrite could make the result negative error.0.1% Sodium nitrite could make the test result decrease by 1.5% relatively.CONCLUSIONS The method is simple,fast and convenient,with accuracy which has met the requirement of hospital infection monitoring and acceptable interfering range made by additives,which is highly practical.
