Aminoglycosides Modifying Enzyme Genes and intⅠ gene in Stenotrophomonas maltoplilia
- VernacularTitle:嗜麦芽寡养单胞菌氨基糖苷类修饰酶与Ⅰ类整合酶基因的研究
- Author:
Xiaowei DENG
;
Danmei ZHANG
;
Changting LIU
;
Hongli ZHOU
;
Ailing LI
- Publication Type:Journal Article
- Keywords:
Stenotrophomonas maltophilia;
Aminoglycosides modifying enzyme;
intⅠ;
Multi-drug resistance
- From:
Chinese Journal of Nosocomiology
2009;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the aminoglycosides modifying enzyme genes and intⅠ gene in Stenotrophomonas maltophilia in Chinese Armed Police Forces General Hospital.METHODS The samples of 27 multi-resistant S.maltophilia were collected from inpatiens from Jan 2006 to Oct 2007 in this Hospital.The sensitivity of the isolates to 14 antibacterial agents was determined using a broth induction method.The aminoglycosides modifying enzyme genes and intⅠ 1 gene were detected by PCR.RESULTS The multi-drug resistance of S.maltophilia was a serious problem.In 27 strains of S.maltophilia,the positive ant(2″)-Ⅰ were in 5 strains(18.5%),aac(3)-Ⅱ in 3 strains(11.1%)and aac(6')-Ⅱ in 1 strain(3.7%).The positive intⅠ gene was found in 11 strains(29.6%).CONCLUSIONS Multi-resistant S.maltophilia resistant to aminoglycosides mainly due to the presence of aminoglycoside modifying enzymes ant(2″)-Ⅰ,aac(3)-Ⅱ and aac(6')-Ⅱ.The aminoglycoside modifying enzymes ant(3″)-Ⅰ and aac(6)-ⅠZ were not detected carrying IntⅠ would be the reason of S.maltophilia resistant to aminoglycosides.
