Characteristics and clinical application of umbilical cord blood stem cells
- VernacularTitle:脐血干细胞的特性及其临床应用
- Author:
Xiaoning TIAN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(36):-
- CountryChina
- Language:Chinese
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Abstract:
Umbilical cord blood that is rich in hemopoietic stem cells and mesenehymal stem cells is collected in vivo and in vitro.Reclamation of stem cells plays an important role in processing umbilical cord blood;additionally,separation of monocytes also plays an important role in establishing bank of umbilical cord blood and performing umbilical cord blood stem cell transplantation.Umbilical cord blood is separated using density gradient centrifugation,hydroxyethyl starch settling process,monoclone antibody flow cytometry,and immunomagnetic bead sorting.Density gradient centrifugation combined with adherent method is used to in vitro separate and culture mesenehymal stem cells;bio-reactor is used to amplify human umbilical cord blood stem cells and progenitor cells.Under a certain condition,mesenehymal stem cells are able to differentiate into hepatocytes.Cryopreservation is mainly used to freeze umbilical cord blood.Vitrification method characterizes by sample,rapid,and high effective,thus it is an ideal method to maintain umbilical cord blood stem cells for a long term.Umbilical cord blood stem cell transplantation benefits for treating various diseases in clinic.
