Effects of AP-2? on invasive growth and ER-? expression of colon cancer cell line
- VernacularTitle:AP-2?对大肠癌细胞侵袭生长及ER-?表达的影响
- Author:
Yeping DU
;
Jinhua MIAO
;
Liping XU
;
Chunmei WU
- Publication Type:Journal Article
- Keywords:
colon neoplasms;
SW620 cell;
transcription factor activator protein-2?(AP-2?);
estrogen receptor-?(ER-?)
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of transcription factor activator protein-2?(AP-2?)on invasive growth and estrogen receptor-?(ER-?) expression in human colon cancer SW620 cells,and to probe into the involved molecular mechanism.Methods: Plasmid pcDNA3.1(+)-AP-2? and pcDNA3.1(+) were transfected into SW620 cells by liposome-mediated transfection.The adhesion,invasion and migration abilities of SW620 cells were measured by metrical gel adhesion assay and modified Boyden chamber(Transwell assay).The gene and protein expression levels of AP-2? and ER-? in SW620 cells were examined by Real-time PCR,Western blotting and immunofluorescence cytochemistry.The interaction between AP-2? DNA and ER-? in SW620 cells was measured by electrophoretic mobility shift assay(EMSA) after AP-2? gene transfection.Results: Overexpression of AP-2? markedly reduced the adhesion,invasion and migration abilities of SW620 cells(all P
