Correlation of Anopheles TEP1 Gene with Melanization Induced by Nitroquine
- VernacularTitle:按蚊TEP1基因与硝喹诱导疟原虫卵囊黑化反应的相关性
- Author:
Jian ZHANG
;
Wenyue XU
;
Jianhua DUAN
;
Fusheng HUANG
- Publication Type:Journal Article
- Keywords:
Nitroquine;
TEP1;
Anopheles stephensi;
Plasmodium yoelii;
Oocyst;
Melanotic encapsulation
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between the TEP1 gene of Anopheles stephensi and melanotic encapsulation of Plasmodium yoelii induced by anti-malaria drug nitroquine. Methods Haemolymph samples from three groups of An. stephensi fed with sucrose solution, Plasmodium-infected blood and nitroquine, respectively, were collected at the 1st, 2nd, 3rd and 4th day after drug adminstration. Degenerate primers were designed according to the conserved amino acid sequence within TEPs of the mosquitoes. Fluorescent quantitation PCR was used to detect the variation of TEP1 gene transcript induced by nitroquine. The melanization of oocysts was observed by light microcopy. Results TEP1 gene was cloned, the predicted amino acid residues harbored a highly conserved canonical thioester motif GCGEQ. The fluorescent quantitation PCR revealed that nitroquine induced an up-regulation of TEP1 activity. The transcription of TEP1 gene in nitroquine treated group (2.423) was significantly higher than that of the infected blood-fed group(1.036) at the 3rd day after nitroquine treatment (P
