Screening and characterization of gastric carcinoma subcell line with higher invasive potential
10.3760/cma.j.issn.1006-9801.2009.01.003
- VernacularTitle:人类胃癌细胞高侵袭亚系的筛选及其生物学特性研究
- Author:
Zhijiao ZHOU
;
Jiansi ZHU
;
Xiang OU
;
Jianguo ZHOU
;
Chengkun WANG
;
Fang PENG
;
Xiyun QUAN
;
Zhixiu ZHOU
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Neoplasm invasiveness;
Transwell;
Screening
- From:
Cancer Research and Clinic
2009;21(1):7-10,13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study a gastric carcinoma subcell line with higher invasive potential and its biologic characteristics screened by a transwell chamber. Methods Transwell chamber was used for the selection of tumor subline. The biological characteristics of the cell lines were studied with optics microscope, Westem blotting, Transwell, immunohistochemical staining and growth curve. Results A gastric carcinoma cell subcell line (named MKN-28S10) was established from its parent cell line MKN-28 with higher invasive potential. MKN-28S10 showed essentially the same morphous as MKN-28. The expression of E-cadherin and TIMP-1 decreased significantly in the screened subcell line(P<0.05). The expression of NM23-H1 in MKN-28S10 was significantly lower than that in MKN-28 (P<0.05). Compared with MKN-28 (61.75±2.06 per vision), the migrative ability of passing through the membrane Millipore(100.25±0.50 per vision) was obviously increased in MKN-28S10(P<0.05). Compared with MKN-28, the growth rate of MKN-28S10 was increased obviously. Conclusion MKN-28S10 cell has stronger invasive ability and more powerful proliferation than that of its mother line MKN-28.