Efficacy of modified PLT additive solution for platelets storage at low temperature(10℃) on rabbit thrombocytopenia model
- VernacularTitle:改良血小板添加液低温(10℃)保存血小板的动物实验研究
- Author:
Xin WANG
;
Ronghua SHI
;
Jing LI
- Publication Type:Journal Article
- Keywords:
Rabbit platelet;
PAS;
Cold-storage;
Trehalose
- From:
Chinese Journal of Blood Transfusion
2008;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the in vivo efficacy of modified platelet(PLT) additive solution(PAS-ⅢM) with trehalose as a substitute for plasma for the storage of platelet concentrates at low temperature(10℃).Methods Rabbit platelet concentrates were collected and stored in different media and temperature conditions,plasma(22℃),70%PAS-ⅢM/30% plasma(10℃) and plasma/trehalose(-85℃).Platelets in plasma(22℃) on storage day 3,in 70%PAS-ⅢM/30% plasma(10℃) on storage day 3,7,9,and in plasma/trehalose(-85℃) thawed on day 20 were transfused to rabbit thrombocytopenia model.The in vivo recoveries and survivals of the three preserved platelets and fresh platelets were measured and evaluated after transfusion into rabit model of thrombocytopenia.Results The survival of platelets stored in 70% PAS-ⅢM/30% plasma(10℃) on day 9 was lower than that of fresh platelets(P0.05).Recovery and survival of frozen platelets were significant lower than those of the other groups(P
