Expression of Toll like receptors on peripheral blood mononuclear cells from patients with idiopathic thrombocytopenic purpura
- VernacularTitle:特发性血小板减少性紫癜外周血单个核细胞Toll样受体表达研究
- Author:
Xiaoliu LIANG
;
Baolong WANG
;
Zhimin SHANGGUAN
- Publication Type:Journal Article
- Keywords:
Idiopathic thrombocytopenic purpura;
Peripheral blood mononuclear cells;
Toll like-receptors;
Real-time quantitative RT-PCR
- From:
Chinese Journal of Blood Transfusion
2008;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the level of the Toll like-receptor mRNA expression on PBMCs from patients with the idiopathic thrombocytopenic purpura.Methods We performed quantitative real-time reverse transcription-polymerase chain reaction(SYBR Green Ⅰ fluorescent dye combination technique)analyses of PBMCs from 30 idiopathic thrombocytopenic purpura(ITP) patients and 30 healthy subjects,to estimate the relative levels of TLR2,TLR4,TLR6,TLR7 and TLR9 mRNA expression.Results Relative expression levels of TLR2 and TLR7 mRNAs in ITP group(n=30)were significantly higher than those in health control group(n=30)(P0.05).Conclusion Expressions of TLR2 and TLR7 mRNA are up-regulated in patients with ITP,which may play an important role in the pathogenesis of ITP.
