Two-way Regulation of Apoptosis-inducing Factor SIVA-1 in the Proliferation and Differentiation of Osteoblasts under Fluid Shear Stress
- VernacularTitle:流体切应力下SIVA-1凋亡诱导因子促成骨细胞增殖分化的双向调节作用
- Author:
Chengjun ZHANG
;
Yayi XIA
;
Changde WANG
- Publication Type:Journal Article
- Keywords:
Fluid shear stress;
Osteoblast;
Proliferation and differentiation;
Apoptosis-inducing factor SIVA-1
- From:Chinese Journal of Minimally Invasive Surgery
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effect of fluid shear stress(FSS) on the proliferation and differentiation of osteoblasts,as well as the expression of apoptosis-inducing factor,SIVA-1.Methods The third-passage osteoblasts were divided into five experiment groups and one control group.In the experiment groups,1.2 Pa FSS were given to the osteoblasts for 0.25,0.5,1,2,and 4 hours respectively,while the control group received no FSS.Afterwards,the cells were harvested to measure MTT value and ALP activity;mRNA level of SIVA-1 were determined by RT-PCR.Results MTT revealed that the cells proliferation markedly increased in the 0.25 h and 0.5 h experiment groups with advanced cell growth curve;whereas significantly inhibited in 1,2,and 4 h groups.The FSS also increased the ALP activity at 0.25 and 0.5 hour,especially in the 0.5 h group(2.4320?0.205 S unit/100ml,158% of the control;P
