Rapid Identification of Staphylococcus aureus in Positive Blood Culture Bottles by Loop-mediated Isothermal Amplification
- VernacularTitle:环介导等温扩增快速检测阳性血培养瓶中的金黄色葡萄球菌
- Author:
Yizheng ZHOU
;
Changfu WANG
;
Xiangyang LI
;
Xiaoyan QIU
;
Qianjun FU
;
Jinhong YANG
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
Meticillin;
Drug resistance
- From:
Chinese Journal of Nosocomiology
2009;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a method of loop-mediated isothermal amplification(LAMP) for detecting Staphylococcus aureus in positive blood culture bottles.METHODS Genomic DNA in 293 positive blood culture bottles was extracted by guanidine hydrochloride and benzenemethanol,then genes ssa and mecA were amplified by LAMP to identify S.aureus.Finally,the results of LAMP were compared with the results of traditional method.RESULTS Twenty-two strains of S.aureus were detected in 293 positive blood culture bottles by LAMP method.Compared with traditional method,the sensitivity and specificity of LAMP method were both 100%,respectively,the detection could be finished in an hour.CONCLUSIONS The LAMP-based assay is simple,rapid,sensitive and specific which can be used to detect S.aureus in positive blood culture bottles rapidly.
