High performance liquid chromatography applied to the comparative study of the fingerprints for different extracts of Vernonia anthelmintica(L.) Willd
- VernacularTitle:高效液相色谱指纹图谱在驱虫斑鸠菊不同提取工艺对比研究中的应用
- Author:
Xiaodong LIU
;
Ming YAN
;
Shixia HUO
;
Lanlan ZHANG
- Publication Type:Journal Article
- Keywords:
Vernonia anthelmintica(L.) Willd;
High performance liquid chromatography;
Fingerprint;
Process comparison
- From:
Journal of Medical Postgraduates
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Vernonia anthelmintica,with complicated components,is a common medicine for the treatment of vitiligo.The purpose of this study was to establish and optimize the separation conditions of high performance liquid chromatography fingerprints(HPLC-FPs) for Vernonia anthelmintica(L.) Willd.Methods: We investigated the HPLC-FPs of different extraction samples by the retention times and relative areas of common peaks in the fingerprints.Results: We established the optimum separation conditions of HPLC-FPs for Vernonia anthelmintica(L.) Willd,and obtained 19 common peaks in the fingerprints.The HPLC-FPs of the 85% ethanol extract were quite similar to those of the semi-bionic extract(SBE) but very different from those of the water extract of Vernonia anthelmintica(L.) Willd.The No.1,4,6,11,12,14 and 16-19 peaks were the endemic ones of the HPLC-FPs of the 85% ethanol extract and SBE of Vernonia anthelmintica(L.) Willd,while No.20-30 were the endemic peaks of the HPLC-FPs of the water extract.Conclusion: The results of our study has provided experimental evidence for further investigation into the constituents absorbed into blood,pharmaceutical technology and pharmacodynamics of Vernonia anthelmintica(L.) Willd.
