5-Azacytidine actives three types of K~+ currents during MSCs proliferating and differentiating processes
- VernacularTitle:5-氮杂胞苷促大鼠MSCs增殖及分化过程中的3种钾电流
- Author:
Ouyang CHEN
;
Gengsheng YU
;
Jie TIAN
;
Hengsheng CHEN
;
Yuan CHEN
;
Yongru QIAN
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
5-azacytidine;
whole-cell patch clamp;
K+ current;
rat
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of the transient outward K+ current (Ito),delayed rectifier K+ current(IkDR) and the inward rectifier K+ current(Ik1) of the rat bone mesenchymal stem cells (MSCs) induced by 5-Azacytidine(5-Aza) during proliferating and differentiating process in vitro. Methods MSCs were cultured according to related articles for two weeks and then some of the cells were induced by 5-Azacytidine.The experiment was divided into uninduced(cultured for 6w) and induced(1,2,3 and 4w) cell groups. Each week had twenty cells random tested by the whole-cell patch clamp technology and the K+ currents were identified by corresponding ionic blockers.Results The detection rate of the same type of K+ current during test weeks had no significant deviation;The three types of K+ current intensity were gradually augmented after being inducing cultured for 1,2,3 and 4 weeks(P
