Expressions of 11?-hydroxysteroid dehydrogenase Types 1 and 2 in colorectal cancer cells and effects of glucocorticoid and glycyrrhetinic acid on this cell line
- VernacularTitle:11?-羟基类固醇脱氢酶在人肠癌细胞株中的表达及甘草次酸的作用
- Author:
Jirong WANG
;
Peng ZHANG
;
Yibing LU
;
Zhaoxia WANG
;
Keming WANG
;
Heng MIAO
- Publication Type:Journal Article
- Keywords:
11?-hydroxysteroid dehydrogenase;
Glucocorticoid;
Colorectal cancer;
Glycyrrhetic acid;
Proliferation
- From:
Journal of Medical Postgraduates
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The 11?-hydroxysteroid dehydrogenase(11?-HSD) plays an important role in tumor biological behavior,and the 11?-HSD inhibitor Glycyrrhetinic acid(GA) may have an anticancer effect,although its mechanism remains unkown.This study aimed to observe the expressions of 11?-HSD 1 and 2 in colorectal cancer and the effects of glucocorticoid and GA on colorectal cancer cells.Methods: The mRNA expressions of 11?-HSD1 and 2 in the human colorectal cancer cell line HCT-8 were detected by reverse transcription polymerase chainreaction(RT-PCR),and the protein expressions of 11?-HSD1 and 2 were detected by Western blot.The inhibition of the growth of the HCT-8 cells was determined by MTT assay after treated with glucocorticoid,cosubstrate,GA only or their combination.Results: The expression of 11?-HSD2 mRNA and proteins but not that of 11?-HSD1 mRNA and proteins was expressed in the HCT-8 cells.The rate of cell growth inhibition was(40.87 ? 1.47)% after a 12-hour treatment with cortisol,and it was(5.79 ? 0.20)% in the cortisol + NAD group,with statistically significant difference(P
