OPN antisense oligodeoxynucleotide inhibits proliferation and apoptosis of human breast carcinoma cell line
- VernacularTitle:骨桥蛋白反义核酸抑制乳腺癌细胞系增殖和诱导其凋亡
- Author:
Yunsong ZHOU
;
Xiaohui WEN
;
Linxi YANG
;
Peng CHEN
- Publication Type:Journal Article
- Keywords:
osteopontin;
antisense oligodeoxynucleotide;
free energy;
breast carcinoma;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects on anti-expression of Osteopontin (OPN) by an antisense oligodeoxynucleotide (ASODN) targeting to low free energy region of OPN mRNA and its effects on the proliferation and apoptosis of human breast carcinoma cell line MCF-7. Methods Designing and synthesizing an ASODN based on minimum free energy algorithm in vitro,which targets to the low free energy region of OPN mRNA. Transfecting it into breast carcinoma cell line MCF-7 which expresses OPN in high level. The cell proliferation-inhibitory rate was determined by MTT method;The morphologic change was observed through transmission electron microscope (TEM);The OPN mRNA expression level was checked by RT-PCR method;Cell cycle and apoptosis rate were detected by flow cytometry (FCM) after transfection respectively. Results OPN ASODN inhibited the proliferation of the cells by a time and concentration dependent manner (P
