Expressions of osteogenesis-related molecules in osteosarcoma cell line MG63
- VernacularTitle:成骨相关分子在成骨肉瘤细胞株MG63中的表达
- Author:
Jianguo ZHAO
;
Weidong XU
;
Zhenhua ZHOU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Osteogenesis-related molecules were detected in osteosarcoma tissues, which have closely association with the occurrence, development and prognosis of osteosarcoma. OBJECTIVE: To study the expression of bone morphogenetic protein 7 (BMP-7), vascular endothelial growth factor (VEGF) and core binding factor ?1 (RUNX2) in osteosarcoma cell Lines MG63. DESIGN, TIME AND SETTING: A molecular biology observation was performed at the Central Laboratory of Changhai Hospital between March 2007 and November 2007. MATERIALS: Osteosarcoma cell line MG63 was purchased from Institute of Biochemistry and cell biology, ISBS, CSA. METHODS: Cell line MG63 was counted with blood cell counting, inoculated in 100 mL culture flask with density of 1?104/cm2, stimulated at 37 ℃ with 5% CO2 air and saturated humidity. Then adherent cultured with DMEM containing 10% fetal bovine serum. When the cells reached 70%-80% confluency, the cells were digested with 0.25% pancreatic enzyme for 5 minutes, followed by terminating the digestion with adding DMEM containing 10% fetal bovine serum, and passaged at a ratio of 1:3. MAIN OUTCOME MEASURES: The expressions of BMP-7, VEGF and RUNX2 in MG63 were detected by RT-PCR and immunohistochemistry. RESULTS: BMP-7, VEGF and RUNX2 were positive expressed in MG63. CONCLUSION: The expression of BMP-7, VEGF and RUNX2 in MG63 was confirmed by gene and protein levels, which suggested that osteogenesis-related molecules may play important roles in the development of osteosarcoma.
