Drug-resistant Genes Associated with Aminoglycosides in Acinetobacter baumannii Isolated from ICU
- VernacularTitle:鲍氏不动杆菌ICU分离株16SrRNA甲基化酶、氨基糖苷类修饰酶基因研究
- Author:
Yiping WANG
;
Guozhong CHEN
;
Jianfei YING
;
Yanhong YU
;
Minfei AN
;
Chengjie ZHOU
;
Mingyang HE
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Aminoglycosides;
Drug-resistant genes;
Aminoglycoside-modifying enzymes;
16S rRNA methylase
- From:
Chinese Journal of Nosocomiology
2009;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the antibiotic resistance of multi-resistant Acinetobacter baumannii(ABA) and distribution of aminoglycoside-modifying enzymes and 16S rRNA methylase genes in ICU in Yinzhou People′s Hospital in Ningbo. METHODS The samples of 20 ABA isolates were collected from Oct 2007 to Jul 2008 in ICU.K-B method was used to determine the sensitivity to 32 antibacterials and the aminoglycoside-modifying enzymes and 16S rRNA methylase genes were analyzed by polymerase chain reaction(PCR). RESULTS From 20 ABA isolates,8 strains carried aminoglycoside-modifying enzymes genes aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb,and ant(3″)-Ⅰ,their positive rate was 10%,15%,30% and 25%,respectively;no strain carried 16S rRNA methylase genes. CONCLUSIONS The antibiotics resistance of A.baumannii is very serious in Yinzhou People′s Hospital in Ningbo.Aminoglycoside-modifying enzymes genes aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb and ant(3″)-Ⅰ exist in multi-resistant A.baumannii widely.They would be the main causes of high drug-resistantce to aminoglycosides.
