Effect of recombinant human granulocyte colony-stimulating factor on the expression of astrocytes in rats with intracerebral hemorrhage
- VernacularTitle:重组人粒细胞集落刺激因子对实验性脑出血大鼠星形胶质细胞表达的影响
- Author:
Xiaoying HE
;
Hua TAN
;
Ping YUAN
;
Xiaogang LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(32):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Astrocytes serve as a major component of central nervous system,which reacted actively to various damages.The astrocytes were strongly expressed with enhanced activity after intracerebral hemorrhage.The pathophysiological significance of this change is presently the research hotspot.OBJECTIVE:To investigate the effects of recombinant human granulocyte colony-stimulating factor(rhG-CSF) on the astrocytes expression after intracerebral hemorrhage.DESIGN,TIME AND SETTING:A randomized control experiment was performed at the Central Laboratory of Luzhou Medical College from March to November 2006.MATERIALS:Fifty healthy,male,SD rats were randomly divided into sham operation(n=10),model(n=20) and experimental(n=20) groups.The rhG-CSF was purchased from Xinpeng Bioengineering Co.,Ltd.METHODS:According to rat brain stereotaxic atlas,intracerebral hemorrhage model was prepared by the method of cutting off tail to obtain blood.The blood was replaced with physiological saline in the sham operation group.Totally 60 ?g/kg rhG-CSF was administered by intraperitoneal injection at 1 hour after model preparation.There was no injection in the other two groups.MAIN OUTCOME MEASURES:The expression of glial fibrillary acidic protein(GFAP) was detected with with ABC immunohistochemical method at hours 6,24,48,72 and day 7 after intervention.RESULTS:There was not GFAP-positive cell found in the sham operation group.The GFAP was little expressed at 6 hours,increased at 48 hours,and reached a peak at 72 hours(P
