Suppressing effect of cadmium chloride on proliferation of hepatocarcinoma
- VernacularTitle:氯化镉对肝癌细胞增殖的抑制作用
- Author:
Wei LI
;
Yehui TAN
;
Yan LI
;
Jing SHEN
;
Xiuli YUAN
;
Guanjun WANG
- Publication Type:Journal Article
- Keywords:
cadmium chloride;
metallothionein;
hepatocarcinoma
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the suppressing effect of cadmium chloride on proliferation of hepatocarcinoma,and to discuss the mechanism for the difference of cadmium chloride between normal and tumor tissue.Methods Hepatocarcinoma cell lines HepG-2 and SMMC-7721 were treated with 5,10,25 and 50 ?mol?L-1 cadmium chloride for 6 h,MTT essay was used to measure the inhibition of proliferation of HepG-2 and SMMC-7721 cells;Instantaneously,HepG-2 and SMMC-7721 cells were treated with 25 ?mol?L-1 cadmium chloride for different time,the inhibition of proliferation was also measured.Athymic mice were used to establish the human hepatocarcinoma animal models,the survival day and tumor volume 8 weeks after drug administration were detected;the metallothionein(MT) levels in normal and tumor tissues were determined by immunohistochemimal staining.Results Compared with control group,5,10,25 and 50 ?mol?L1 cadmium chloride inhibited the proliferation of hepatocarcinoma cell lines(P
