Abnormality of multilineage difierentiation potential of bone marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus
10.3760/cma.j.issn.1007-7480.2009.03.013
- VernacularTitle:系统性红斑狼疮患者骨髓间充质干细胞多向分化能力异常
- Author:
Ting XU
;
Ouyang JIN
;
Shengnan ZHAO
;
Huayong ZHANG
;
Yayi HOU
;
Lingyun SUN
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Mesenchymal stem cells;
Cell differentiation
- From:
Chinese Journal of Rheumatology
2009;13(3):185-188,插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the muhilineage differentiation potential of bone marrow-derived mesenchymM stem eels (MSCs) in patients with systemic lupus erythematosus (SLE).Methods Density gradient centrifugation and plastic adherence methods were used for isolation of marrow-derived MSCs.Then tIIeir differentiation potentiality to lipoblasts and osteoblasts waft tested.MSCs loading on hydroxyapatite were elnbedded in the nude mouse's subcutaneous tissues.Eight weeks later.osteogenesis was evaluated by HE staining.PPA Rγ2,LPL,Runx2/CBFA1,osteocalcin gene expression in MSCs after differentiation were examined by RT-PCR.Results The positive rates of lipoblasts stained by oil red O and optical density in SLE were decreased than in the control group[(35±7)% vs (80±5)%] (0.14±0.04 vs 0.27±0.04),and the positive rates of osteoblasts stained by Alizarin Red S in SLE were decreased than those in the control group [(35±4)% vs (45±4)%].Osteoblast differentiation in the SLE group was less than that of the contro]group.The mRNA expression of LPL (0.369±0.020 vs 0.481±0.038).Runx2/CBFA1 (0.371±0.000 vs 0.563±0.069).osteoealcin (0.819±0.023 vs 0.962±0.049) of MSCs after difierentiation in the SLE group was decreased than that of the control group.There was no significant difference in the expression of PPARγ2 mRNA between SLE and controI group (0.421±0.052 vs 0.441±0.012).Conelusion MSCs from SLE have abnormalities in osteogenie and adipogenic differentiation potential.
