Immuno-PCR Assay on Detection of HIV-1 p24 Antigen:A Primary Evaluation
- VernacularTitle:免疫PCR检测HIV-1 p24抗原的初步评价
- Author:
Ji ZHENG
;
Weiling FU
;
Tianlun JIANG
- Publication Type:Journal Article
- Keywords:
Immuno-PCR;
p24;
Limit;
Specificity;
Reproducibility
- From:
Chinese Journal of Nosocomiology
2009;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate primarily the detection limit,specificity and reproducibility of immuno-PCR assay on HIV-1 p24 antigen.METHODS We p24 antigen were detected by established immuno-PCR system,and then the detection limit,specificity and reproducibility were discussed.We quantitatively analyzed the nonspecific amplified bands with fluorescence intensity(FI),and a preliminary determination of the lower specific amplification limit was made.RESULTS We taken(x-+3s)FI of nonspecific amplification as the lower limit of specific amplification signal.The detection limit of immuno-PCR assay was 0.1 ng/L.CONCLUSIONS The detection limit,specificity and reproducibility can meet the needs of HIV-1 p24 antigen detection.
