IFN-? Release Assays for Rapid Diagnosis of Active Tuberculosis:Clinical Application
- VernacularTitle:结核分枝杆菌?-干扰素体外释放定量试验诊断结核感染的临床应用
- Author:
Hua ZHANG
;
Shengwen HUANG
;
Zhenyuan LUO
- Publication Type:Journal Article
- Keywords:
IFN-? release assays;
Mycobacterium tuberculosis;
Diagnosis
- From:
Chinese Journal of Nosocomiology
2009;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the clinical application of T-cell based IFN-? release assays(IGRA) for the rapid diagnosis of active tuberculosis.METHODS IFN-? and HIV antibody were detected by using ELISA.Antibody to Mycobacterium tuberculosis was detected by colloidal gold.At the same time,the M.tuberculosis DNA loads were examined by FQ-PCR.Statistical analysis were performed to analyze the correlation of IFN-? with M.tuberculosis antibody and DNA,respectively.RESULTS The sensitivity of TB-IGRA was 90.24%,specificity was 93.34%;the positive rate of TB-IGRA in 82 tuberculosis patients was higher than from sputum smear(64.63%),TB-PCR(76.83%) and tuberculosis antibody(40.24%).CONCLUSIONS As a replacement of TB-PCR,IFN-? can be used as a valued index to evaluate tuberculosis infectin.
