Detection of Carcinoma of Urinary Bladder by Fluorescence in situ Hybridization
- VernacularTitle:荧光原位杂交技术在膀胱癌诊断中的应用
- Author:
Guangjie WANG
;
Xiaoli ZHANG
;
Zhiwen CHEN
;
Yang LUO
;
Weiling FU
- Publication Type:Journal Article
- Keywords:
Fluorescence in situ hybridization;
Bladder neoplasms;
Exfoliated cell
- From:
Chinese Journal of Nosocomiology
2009;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the molecular cytogenetic alterations of transitional cell carcinoma of the urinary bladder with exfoliated cells by fluorescence in situ hybridization(FISH) analysis of chromosome-specific probes.METHODS FISH was performed using 3,7,9 and 17 of chromosome-specific probes to examine chromosome aberration of exfoliated cells in 50 urine samples from patients with transitional cell urinary bladder carcinoma.RESULTS The frequency of numerical aberration of chromosomes 3,7,9 and 17 was 28%,32%,56% and 38% in urinary exfoliated cells,respectively.Loss of chromosome 9 was the most common finding,but it was not correlated with pathological grade of cancer and stage of the disease.Abnormality of chromosomes 3,7 and 17 was associated with the clinical stage.CONCLUSIONS A number of chromosome aberrations are detected in transitional cell carcinoma of the urinary bladder by FISH technique which provides a basis for further understanding of its molecular pathogenesis.It is a rapid,accurate and very sensitive method and can be used in clinical diagnosis.
