Possibility of Methylation Profile of Estrogen Receptor Gene Promoters as an Early Diagnosis Biomaker in Leukemia
- VernacularTitle:雌激素受体启动子异常甲基化作为白血病早期诊断分子标志物的实验研究
- Author:
Jie YAO
;
Xiaobing ZHANG
;
Weiling FU
- Publication Type:Journal Article
- Keywords:
Leukemia;
Estrogen receptor;
DNA methylation;
Western-blot
- From:
Chinese Journal of Nosocomiology
2009;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the methylation status and mRNA expression of the estrogen receptors(ERs) gene promoter in acute leukemic patients and detect the protein expression in leukemia cell lines with or without treatment of 5'-aza-Dc.And to find out the possibility of promoters methylation profile of estrogen receptor gene as an early diagnosis biomarker in leukemia.METHODS With RT-PCR and MSP,evaluating ERs mRNA expression and status of methylation in 40 acute leukemia patients without treatment.With Western-blot,detecting protein expression in leukemic cell lines with or without treatment of 5-azaDc.RESULTS The protein expression was significantly enhanced in all of leukemic cell lines with 5'-Aza-Dc.ER?-A was inactivated and specifically methylated(97.5%;39/40) in most of the acute leukemic patients.CONCLUSIONS The promoter ER?-A is inactivated and specifically methylated(97.5%;39/40) in most of the acute leukemic patients.This study may provide a new direction method to study the pathogenic mechanism of leukemia,and indicates that ER?-A methylation could be a potential reference marker for leukemia diagnosis.
