TSA promotes mesenchymal stem cells differentiating into cardiomyocyte-like cells
- VernacularTitle:曲古抑菌素A促进大鼠骨髓间充质干细胞向心肌样细胞分化
- Author:
Lili ZHAO
;
Jing ZHU
;
Chuan FENG
;
Ge YANG
;
Jie TIAN
- Publication Type:Journal Article
- Keywords:
histone deacetylase inhibitors;
mesenchymal stem cells;
diferentiation;
cadiocyte
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of trichostatina to induce mesenchymal stem cells(MSCs) differentiating into cardiomyocyte cells and to explore the potential interference approaches.Methods MSCs were incubated with different concentrations of TSA and different time,then to detecte the histone deacetylase(HDAC) activity;the differentiation of MSCs was observed by phase-contrast mictmcopy;fluorescence immunohistochemistry assessed cardiac troponin T and RT-PCR detected the expression of Nkx-2.5,GATA-4 genes.Results HDAC activity was the lowest when incubated with 300 nmol/L TSA for 24 hours,expression of cTnT of the cells was enhanced after incubation with TSA.The expression of Nkx-2.5,GATA-4 genes was enhanced before interference.ConclusionTSA can promote MSCs to differentiate into cardiomyocyte cells in vitro in an optimized condition.
