Human bone morphogenetic protein 3(hBMP3) inhibits growth of human osteosarcoma cells MG63 and U2OS
- VernacularTitle:人骨形态发生蛋白3抑制骨肉瘤细胞系MG63和U2OS的体外生长
- Author:
Limei WU
;
Huanling HE
;
Xingxing LI
;
Yinghua CHEN
;
Jia WEI
;
Guowei ZUO
;
Jingkun MIAO
;
Yan WANG
;
Lan ZHOU
- Publication Type:Journal Article
- Keywords:
bone morphogenetic protein;
osteosarcoma;
proliferation;
apoptosis;
transmigration
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The biological effects of BMP3 on osteosarcoma were investigated by treating human osteosarcoma cell lines MG63,and U2OS with human BMP3(hBMP3).Methods Osteosarcoma cells in experimental groups were respectively treated with AdBMP-3 and rhBMP3-CM,control groups with AdGFP and rGFP-CM,the blank group with neither.Their ability of proliferation,apoptosis,transmigration and differentiation were respectively detected by trypan blue exclusion test,terminal deoynucleotidyl transferase mediated dUTP nick end labeling(TUNEL) and acridine orange-ethidium bromide fluorescent stain(AO/EB),transwell-room and alkaline phosphatase(ALP) activity reagent kit.Results(1) All the indexes detected were not significantly different between two control groups.(2) Compared with control groups,the cell survival rate showed a significant decrease in experimental groups.(3) The apoptosis indexes increased.(4)The number of trans-membrane cell decreased.(5)The activity of alkaline phosphatase increased after treatment with AdBMP3 and rhBMP-3 for 3 days in MG63,5 days in U2OS.Conclusion hBMP3 inhibit osteosarcoma cells growth and promote bone formation.
