Functional Expression of Human Cytochrome P450 Enzymes in Escherichia coli
- VernacularTitle:人细胞色素P450在大肠杆菌中的功能表达
- Author:
Dacheng HAO
- Publication Type:Journal Article
- Keywords:
Cytochrome P450 NADPH-P450 oxidoreductase Heterologous expression Escherichia coli Expression optimization
- From:
China Biotechnology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Functional expression of human cytochrome P450(P450,CYP)in E.coli is important for new drug R&D,clinical drug therapy and the study of early ADME/T properties.Escherichia coli is the most extensively utilized host in the production of recombinant human P450 enzymes.However,it is challenging to obtain sufficient P450s with catalytic activity.Depict current developments in the heterologous expression of human P450 enzymes,strategies for the expression of human P450s in E.coli,factors affecting high-level expression,and coexpression.The recent works of authors in expression optimization are also reviewed.
