Generation of a Recombinant Baculovirus Expressing The E2 Protein of Classical Swine Fever Virus and Its Immunogenicity in a Mouse Model
- VernacularTitle:表达猪瘟病毒E2蛋白的BacMam病毒的构建及其免疫原性分析
- Author:
Miao LI
;
Yufei WANG
;
Yu WANG
;
Hui GAO
;
Na LI
;
Yuan SUN
;
Bingbing LIANG
;
Huaji QIU
- Publication Type:Journal Article
- Keywords:
classical swine fever virus, E2 gene, recombinant baculoviruses, immunogenicity
- From:
Progress in Biochemistry and Biophysics
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Baculovirus-mediated gene transfer into mammalian cells has been used to develop non-replicative vector vaccines against a number of diseases in several animal models.A baculovirus pseudotyped with the glycoprotein of vesicular stomatitis virus was used as vector to construct the recombinant baculovirus expressing classical swine fever virus(CSFV) E2 protein under the control of ie1 promoter from white spot syndrome virus.The E2 gene was shown to be efficiently expressed in both insect and mammalian cells.Intramuscular injection of mice with the recombinant baculovirus resulted in the production of high-level CSFV-specific antibodies.Specific lymphoproliferative responses to the CSFV stimulation were induced in the splenocytes of the immunized mice as demonstrated by CFSE staining assay and WST-8 assay.The results indicates that the pseudotyped baculovirus-delivered gene can be a potential non-replicative vaccine against CSFV infection.
