Two Laboratory Detections of Epstein-Barr Virus:A Comparative Analysis
- VernacularTitle:EB病毒两种实验室检测方法对比分析
- Author:
Guohua ZHUANG
;
Biao LI
- Publication Type:Journal Article
- Keywords:
Epstein-Barr virus;
Immunofluorescence technique;
Fluorescence quantitative polymerase chain reaction;
Diagnosis
- From:
Chinese Journal of Nosocomiology
2009;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To compare the effectiveness of two laboratory detections methods of Epstein-Barr virns (EBV). METHODS Immuno-fluorescence technique was applied to detect the serum EBV-specific antibody and EBV-DNA was identified by fluorescence quantitative polymerase chain reaction (FQ-PCR) in 124 children infected with EBV. RESULTS The positive rates of VCA-IgM,VCA-IgG,EA-IgG,EBNA-1-IgG and EB-DNA in various samples of 124 cases were 0,89.5%,23.4%,49.2% and 30.3%,respectively,with 21 cases (16.9%) showing simultaneously positive in EB-DNA and EBNA-1-IgG. CONCLUSIONS The two laboratory detections of EBV share their advantages and disadvantages. FQ-PCR is a method for time-saving,accurate and sensitive detection of EBV-DNA,showing clinical significance in the diagnosis of EBV-associated diseases. It's worthy of clinic application.
