Effects of calcitonin gene-related peptide on osteoprotegerin and RANKL expressions in osteoblast cells in bone metastasis microenvironment of breast cancer in vitro
- VernacularTitle:模拟乳腺癌骨转移微环境中CGRP对成骨细胞OPG和RANKL表达影响
- Author:
Chen YANG
;
Hui ZHAO
;
Yang YAO
;
Zhiyu WANG
;
Ping CHEN
- Publication Type:Journal Article
- Keywords:
calcitonin gene-related peptide(CGRP);
breast cancer cell;
osteoblast cell;
osteoprotegerin(OPG);
receptor activator of nuclear factor-kappaB ligand (RANKL)
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of calcitonin gene-related peptide (CGRP) on the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-kappaB ligand (RANKL) in osteoblast cells through an in vitro breast cancer cell and osteoblast cell co-culture system. Methods:The metastatic breast cancer MDA-MB-231 or MDA-MB-435 cells were co-cultured with osteoblast MG63 cells to establish an in vitro microenvironment of bone metastasis of breast cancer. After treated with CGRP(1?108 mol/L),OPG and RANKL mRNA and protein expressions in osteoblast MG63 cells were examined by RT-PCR and Western blotting. Results:Expression of RANKL in osteoblast MG63 cells was up-regulated at both mRNA and protein levels when osteoblast MG63 cells were co-cultured with breast cancer MDA-MB-231 or MDA-MB-435 cells,while those of OPG in osteoblast MG63 cells were both down-regulated (P
