Serum proteomic spectra of esophagial carcinoma patients and a corresponding esophagial carcinoma diagnostic model
- VernacularTitle:食管癌患者血清蛋白指纹图谱的检测及食管癌诊断模型的建立
- Author:
Lihua LIU
;
Baoen SHAN
;
Shijie WANG
;
Jun MENG
;
Ling WANG
- Publication Type:Journal Article
- Keywords:
esophagial neoplasms;
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;
serum proteomic pectra;
diagnostic model
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examine the serum proteomic spectra of human esophagial carcinoma by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS),so as to set up a diagnostic model of esophagial carcinoma and to investigate its clinical value. Methods:Thirty-two esophagial carcinoma patients and 28 healthy controls were obtained from Fourth Affiliated Hospital of Hebei Medical University during May to September of 2008. Serum protein was extracted by weak cation exchange (WCX) protein chip system,and proteomic spectra was examined by MALDI-TOF MS. The obtained data were analyzed by ZUCI-protein chip data analyze system (ZUCI-PCDAS) and an esophagial carcinoma diagnostic model was established by genetic arithmetic (GA) combined support vector machine (SVM). The above 60 samples were randomly divided into training set and blinding test set,with training set including 21 esophagial carcinoma patients and 19 healthy controls and blinding test set including 11 esophagial carcinoma patients and 9 healthy controls,so as to examine the specificity and sensitivity of this diagnostic model. Results:Serum proteomic spectra of esophagial carcinoma patients and healthy controls were obtained by MALDI-TOF MS,and m/z (mass to charge) peaks of 44 differential proteins were obtained after analyzed by ZUCI-PCDAS software package (P
